Products specifications
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Antigen
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Enolase, Non Neuronal (NNE)
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Reactivity
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Human
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Host
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Rabbit
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NCBI Accession
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RPB449Hu02
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Concentration
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200ug/ml
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Conjugate
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No Conjugate
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Immunogen
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Recombinant NNE (Met1~Lys434) expressed in E.coli.
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Immunoglobulin Type
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IgG
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Purification
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Affinity Chromatography
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Specificity
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The antibody is a rabbit polyclonal antibody raised against NNE. It has been selected for its ability to recognize NNE in immunohistochemical staining and western blotting.
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Application
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WB, ICC, IHC-P, IHC-F, ELISA
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Amino Acid Sequence
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MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
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Shipping Conditions
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2 °C - 8 °C
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Storage Temperature
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Store at 4 °C for frequent use. Store at -20 °C to -80 °C for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Handling Instruction
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Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Notes
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All antibodies are custom made on-demand. Please allow 5-7 working days for production and 2-3 days for shipment.
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Legal Information
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Marketed under license from USCN Life Science Inc.
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Optimal working dilutions must be determined by end user.
| Western blotting | 1:100-400 |
| Immunocytochemistry in formalin fixed cells | 1:100-500 |
| Immunohistochemistry in formalin fixed frozen section | 1:100-500 |
| Immunohistochemistry in paraffin section | 1:50-200 |
| Enzyme-linked Immunosorbent Assay | 1:100-200 |